Synthetic materials alternatives are crucial for reaching sustainable development goals and waste reduction. Biomaterials and biomolecules obtained through bacterial fermentation offer a viable solution. Double-layer active UV-blocking material composed of bacterial nanocellulose as an inner layer and poly(hydroxyoctanoic acid) containing prodigiosin as an active compound was produced by layer-by-layer deposition. This study referred the new material consisted of the three components produced in sustainable manner, by bacterial activity: bacterial bio-pigment prodigiosin, bacterial nanocellulose and poly(hydroytoctanoate) - biopolymer obtained by microbial fermentations. Prior the final double layer film was produced, PHO films containing different PG concentrations as a layer in charge of the bioactivity (0.2, 0.5 and 1 wt%) was casted and systematically characterized (FTIR, DSC, XRD, wettability, SEM, transparency, mechanical tests) to optimize their properties. The formulation with the best UV-blocking properties and less toxicity effect tested using MRC5 cells was chosen as an outer layer in double-layer films production. Water contact angle measurements confirmed that hydrophilic – hydrophobic double layer film was obtained with the improved mechanical properties in comparison to the native BNC. Migration test indicated release of PG in all tested media as a consequence of bilayer formulation, while the PG release from PHO in 10% ethanol was not detected. All findings from the study suggested this activated, UV-blocking material as a candidate with excellent potential in packaging industry.

Double layer bacterial nanocellulose - poly(hydroxyoctanoate) film activated by prodigiosin as sustainable, transparent, UV-blocking material

This study explored the biodegradation potential of microbial isolates focusing on their ability to utilize biopolymers as sole carbon source. Previously described isolates have been investigated through agar-based screen for the ability to degrade plastic-related substrates in powder form, and four strains have been selected for further assessment. Polyhydroxybutyrate (PHB) films degradation was examined through liquid culture, soil burial and respirometry assays. Structural and chemical alterations in PHB were analysed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). The most successful strains were tested for the ability to degrade PHB/bacterial nanocellulose (BNC) blends. 

 sp. DG90 excelled in PHB degradation, achieving 60% weight loss in liquid culture, while 

 sp. DG19 exhibited a notable degradation rate of 51 ± 1.7%. Soil burial assays underscored the impact of environmental factors on degradation rates, emphasizing the role of soil composition and nitrogen availability. In respirometry assay, PHB films were severely defragmented by 

 sp. DG19 with overall weight loss of 83%, while for 

 sp. DG90, this percentage reached 39%. FTIR and DSC analyses suggested potential hydrolysis and structural alterations in treated samples. This study observed rapid PHB degradation (83% in 3 weeks) while, considering the complex composition of modern biomaterials, also showcased the potential of examined strains to degrade PHB-BNC blends up to 85%.

Unveiling the potential of bacterial isolates from plastic-polluted environments: enhancement of polyhydroxybutyrate biodegradation

Technology's influence on our lives and the world has reached unprecedented levels. Its advancements affect both humanity and the environment, underscoring the necessity to harness it for addressing global issues. By focusing on environmental protection, green transition, sustainable development, healthcare, agriculture, energy, climate change, and more, we can steer technological progress towards positive outcomes.

International Fair of Technics and Technical Achievements is a traditional meeting and business contact point, which enables numerous exhibitors and visitors to satisfy their business expectations. This is the most important economic and technological event in Serbia and the South East Europe.

Representatives from IMGGE: Dr Sanja Skaro Bogojevic, Dr Jelena Milovanovic, Dr Jelena Lazic

66th International Fair of Technics and Technical Achievements

 in Belgrade (Serbia) was organized in 7 halls of 14,840 m2 and 1 open space of 200 m2, where 579 national and 325 international exhibitors from 30 countries presented cutting-edge technologies from the fields of technics, engineering, industry and innovations. Over the 4 days of the Fair, the team from the Institute of Molecular Genetics and Genetic Engineering (IMGGE) presented their research projects under the slogans “Microorganisms: ready to face great challenges” and “Creative paths of waste” to 19,047 curious visitors of all ages.

Representatives from IMGGE: Dr Milica Ciric, Milena Stevanovic, Dr Vuk Filipovic

Representatives from IMGGE: Dr Sandra Vojnovic, Dr Tatjana Ilic-Tomic, Dr Marijana Ponjavic

The EcoPlastiC project was at the center of it all, where various tangible bioplastic materials (PHA, PLA) and bacterial nanocellulose (solubilized or dry) were presented as possible solutions of the worldwide problem of plastic waste.

The EcoPlastiC project also attracted media’s attention, so our colleague Dr Sanja Skaro Bogojevic gave a statement for the EKOVIZIJA television, the first Serbian TV channel dedicated especially to ecology. The full interview for ECO REPORTAGE is available here: 

https://www.youtube.com/watch?v=8gauN4u2Tbs

EcoPlastiC at the 66th International Fair of Technics in Serbia